Molecular identification of Theileria spp in cattle from Iwo Osun state, Nigeria
نویسندگان
چکیده
Abstract Theileria is an haemoparasitic protozoa causing disease known as theileriosis in animals. The transmitted by tick vectors. circulates cattle and causes the dairy industry to suffer significant financial losses it affects milk livestock production, especially sub-Saharan region. endoparasite can exist sub-clinical state that are carriers, these transmit diseases become reservoir of infection for ticks. In Nigeria, diagnosis this parasite has been done mainly through light microscopy Giemsa stain; molecular report scanty. Hence, work was identify characterise species infecting healthy Iwo Osun state, Nigeria utilizing genetic approach (PCR 18S rRNA gene sequencing). cross-sectional investigation, blood drawn aseptically from cows’ jugular veins using a sterile needle syringe. Whatmann filter paper No. 3 spotted with blood. Cloning sequencing three samples successfully amplified. Phylogenetic analysis showed two comprising one T. velifera mutans . outcome will help decision-making process developing control measures cows Nigeria.
منابع مشابه
Analysis of Agro-Chemical Inputs Use in Maize Production among Small-Scale Farmers in Iwo Local Government, Osun State, Nigeria
In spite of the development of various improved cultivars of maize, yield in Nigeria has continued to be hampered by the over cropped nature of most farmlands as well as a wide range of pests and diseases. This study therefore carried out an analysis of agro-chemical inputs’ use in maize production among small scale farmers in Iwo Local Government Area of Osun State. Specifically, the study inv...
متن کاملMolecular and Microscopic Detection of Theileria spp. among Cattle and Buffaloes in West Azarbaijan, Iran
Bovine theileriosis is an important tick-borne disease caused by intraerythrocytic parasites from genus Theileria. This study sought to detect the theileriosis among cattle and buffaloes using molecular and microscopic tests in West Azerbaijan, Iran. For this purpose, 484 blood samples from 193 cattle and 291 buffaloes were collected during March to July 2014. The breed, gender, age, and habita...
متن کاملSarcocystis cruzi: First molecular identification from cattle in Iran
Sarcocystis is a genus of cyst-forming parasites infecting both animals and human. This study aimed to isolate coccidian tissue cysts from muscle of infected animals by a simple method in addition to molecular identification of Sarcocystis cruzi from the samples. The samples were obtained from commercial source in Babol, Northern Iran. Five grams of calf muscle was cut into 2-3 pieces in 30 ml ...
متن کاملanalysis of agro-chemical inputs use in maize production among small-scale farmers in iwo local government, osun state, nigeria
in spite of the development of various improved cultivars of maize, yield in nigeria has continued to be hampered by the over cropped nature of most farmlands as well as a wide range of pests and diseases. this study therefore carried out an analysis of agro-chemical inputs’ use in maize production among small scale farmers in iwo local government area of osun state. specifically, the study inv...
متن کاملIsolation and Molecular Identification of Mycoplasma spp. From Pigeons in the North-East of Iran
Background: Mycoplasma is one of the most important pathogens of respiratory system in poultry. OBJECTIVES: The aim of the present study was to identify Mycoplasma spp. isolated from pigeons. METHODS: Sixteen pooled samples were provided and cultured on PPLO medium and finally the DNA was extracted from the resulting single colonies. RESULTS: Through 16S rRNA gene amplification Mycoplasma genus...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: IOP conference series
سال: 2023
ISSN: ['1757-899X', '1757-8981']
DOI: https://doi.org/10.1088/1755-1315/1219/1/012006